Even rubber bands are unnecessary. For larger manuscripts, loose pages neatly inside a box of proper size is enough.* The easier it is for an agent to handle the pages, the better.
Yes. In the US, screenplays are three-hole punched, but only two brads are used, never three. And the color of the cover signifies what version of the screenplay the script is.*The exception being movie/TV scripts, which have their own preferred, and fairly rigid, submission format.
And for future reference, don't over package a submission. For a full manuscript just wrap it neatly with paper (similar to how copy paper comes packaged), put rubber bands around it, and put it in a box. Don't use bubble wrap and tape it all together and put it in a smaller box and put more bubble wrap around that, etc, etc.

You could invite all the neighborhood kids over and have a bubble popping party.There goes my closet full of bubble wrap!
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Really!? You just put them loose in a box? Ok. Where do you put the cover letter? Just on the top of it all?
Rubber bands aren't really that complicated to use.
And there's nothing worse than opening up a loose manuscript and something going wrong (butterfingers!) so all those loose, unbound pages go flying....
All I know for sure is that whenever my editor sends a bound, edited manuscript back, it's got at least one, sometimes two, rubber bands holding it together. So some industry folks do use them.![]()