Quote Originally Posted by Enlightened View Post
If it is not the only problem, or just one of a few, small things, likely not. If either (or both) of the matters are part of a list where these tip it into the pass category, it's something to consider for the OP.
I know and have known a LOT of agents and editors.

I don't know a single one who would reject a book because of a punctuation issue like this.

It's a small thing. It's better to get punctuation right, of course: but a misplaced quote-mark is not a matter for rejection.