Con'd
This is straight from Glimmer Train Stories FAQ's. (This same FAQ was there when they were still accepting poetry.) It covers a multitude of sins, but remember this is just one pub. I'm pasting it because I think it gives some insight into first rights problems:
<<You only accept “unpublished” work. Define that please.
“This can be a tough one. If you’ve made 50 copies of your manuscript for classmates and friends, for instance, we'd call that unpublished. Heck, even if you had Kinko's bind them, we'd go for that. But if someone else put it out--say the school newspaper, or your writing group, we would look at that as published, especially if more than one piece of work (all yours, or yours and someone else's) was included in the pages. (Fifty copies of a collection of people’s writing in it just about qualifies as a “litmag” these days.) And if there is ANY advertising, no matter the number of copies printed, it will be considered “previously published” by anyone I know.
Posted “only on a website”? That is generally okay by us. Our concerns are twofold: 1) That the large majority of our readers not have previously read a story we print, and, 2) That you have the right to offer us first-print publication rights.
We reserve the right to request a copy of the distributed piece or access the spot where it resides before deciding if the piece seems “published” to us.>>